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. Author manuscript; available in PMC: 2008 Oct 2.
Published in final edited form as: Dev Dyn. 2008 May;237(5):1321–1333. doi: 10.1002/dvdy.21526

Figure 5. RA induces Cyp26b1 and represses Nr2f2 in male mouse UGS organ culture.

Figure 5

UGS from E14.5 male C57BL/6J mouse fetuses were incubated for 24 h in organ culture media containing vehicle or RA (0-10 μM). Abundance of Cyp26b1 and Nr2f2 was determined by real-time RT-PCR and normalized to the mRNA abundance of the housekeeping gene peptidyl prolyl isomerase a (Ppia). Results are mean ± SEM of n ≥ 4 independent samples per group from at least 3 separate litters. Significant differences among groups were determined by ANOVA followed by Fisher’s Least Significant Difference test. The presence of an asterisk indicates significantly different from vehicle (open bars, p ≤ 0.05). The presence of a dagger indicates significantly different from 10 μM RA (p ≤ 0.05).