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. Author manuscript; available in PMC: 2008 Oct 2.
Published in final edited form as: Dev Dyn. 2008 May;237(5):1321–1333. doi: 10.1002/dvdy.21526

Figure 8. SHH and NOGGIN induce prostatic bud formation in the absence of RA.

Figure 8

UGS from E14.5 male C57BL/6J mouse fetuses were incubated for 3 d in organ culture media containing vehicle, recombinant SHH protein (5 μM), recombinant NOGGIN protein (1 ug/ml)or a combination of SHH + NOGGIN. Media and recombinant proteins were replenished daily. At the end of the incubation, UGE was separated from UGM as described in Methods and visualized by SEM. Prostatic buds were counted using micrographs of each UGS that were taken from four separate angles in order to observe the entire UGS epithelial surface and ensure that all prostatic buds present were counted. Results for the total number of prostatic buds per UGS are expressed as the mean ± SEM of n ≥ 6 independent samples per group for at least 3 separate litters. Significant differences among means were determined using one-way ANOVA, followed by Fisher’s LSD post-hoc test. The presence of an asterisk indicates a mean that is significantly different from the vehicle (control) mean (p ≤ 0.05).