Figure 6.

Adenylyl cyclase activity of wild-type, gβ⁻, and SN mutant cells. Cells were developed to the aggregation stage, and adenylyl cyclase activities in the lysates of wild-type, gβ⁻, SN1, SN2, SN3, SN4, and SN5 cells were measured in buffer (open bars), in the presence of Mn²⁺ (shaded bars), and in the presence of GTPγS (black bars). Means and SEMs of an experiment done in duplicate are presented. At least one independent experiment was done for each cell line and yielded similar results.