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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: J Proteome Res. 2007 Sep 28;6(11):4230–4244. doi: 10.1021/pr070313u

Figure 3. ERPA (CID / ETD) analysis of a monophosphorylated peptide (3+ charge state) from the Lys-C digest ofβ-casein.

Figure 3

A: Base peak ion chromatogram. B: CID-MS2 spectrum of the m/z 1688.76 (3+) ion eluted at 35.04 min. C: ETD-MS2 spectrum of the m/z 1688.76 (3+) ion eluted at 35.05 min. D: CRCID-MS3 scan of the m/z 1031.74 ion isolated from the ETD spectrum, as indicated by the dotted circle. The peptide sequences with the observed fragment ions are shown in the insert: phosphoserine is indicated as pS. The neutral loss of phosphate is also shown.