Skip to main content
. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: J Proteome Res. 2007 Sep 28;6(11):4230–4244. doi: 10.1021/pr070313u

Figure 8. ERPA (CID / ETD) analysis of an O-linked glycosylated peptide fragment modified with fucose from the Lys-C digest of t-PA.

Figure 8

A: Base peak ion chromatogram. B: CID-MS2 spectrum of the m/z 1004.18 ion. C: ETD-MS2 spectrum of the m/z 1004.18 ion. D: CRCID-MS3 scan of the m/z 1339.17 ion (from the ETD spectrum as indicated by the dotted circle). The peptide sequences with the observed fragment ions are shown in the inserts. The fucosylation site is labeled T - Fu. The loss of fucose (-Fu) from the fragmentation in CID is indicated in panel B. The sequence tags of FGE and QQALYFS are indicated in bold in the inserts of panels C and D, respectively.