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. Author manuscript; available in PMC: 2008 Oct 2.
Published in final edited form as: Oncogene. 2007 Nov 12;27(19):2754–2762. doi: 10.1038/sj.onc.1210925

FIG. 2. H-Ras is a minor isoform in HeLa cells compared to K-Ras and N-Ras.

FIG. 2

(A) Isoform-specific Ras knock-down using RNAi; >90% knock-down of each isoform was achieved. Probing the knock-down lysates with the anti-pan-Ras antibody Y13-259 enables the relative abundance of each isoform to be determined. (B) Y13-259 as a true pan-Ras antibody. Lysates normalised for GFP expression from HeLa cells expressing GFP-tagged Ras isoforms were probed with a variety of anti-pan-Ras antibodies. Only Y13-259 exhibited no isoform preference.