Figure 2.

Truncation of U7 snRNA by an antisense oligodeoxynucleotide. Xenopus oocytes were injected with an antisense U7 or a control oligonucleotide. After 2 h or 18 h incubation at 19°C, RNA was purified from single GVs, separated on a polyacrylamide gel, and hybridized with anti-U7 and anti-U2 snRNA probes (ratio 1000:1). Truncated U7 is detected at 2 h, corresponding to loss of 16 nucleotides from the 5′-end. The truncated product was unstable and was not detected at 18 h. U2 snRNA was unaffected by either the anti-U7 or the control oligonucleotide.