Figure 1.

Gangliosides inhibit cross-linking of GH-DAF in MDCK cells. MDCK GH-DAF cells were incubated with 0, 10, 50, and 100 μM GM₁ (A) or with 10, 50, and 100 μM bbG (B) for 1 h at 37°C. Cells were subsequently subjected to cross-linking with BS³. Proteins were resolved on 5–15% SDS-PAGE and after Western blotting were detected with anti-GH antibody followed by ECL. Western blots were scanned, and the intensity of immunoreactive signal is given as relative optical density (ROD) for 0 μM GM₁ (A, bottom curve), 0 μM bbG (B, bottom curve), 100 μM GM₁ (A, top curve), and 100 μM bbG (B, top curve).