Figure 7.

Ganglioside treatment does not change the immunofluorescence pattern of GH-DAF. MDCK GH-DAF cells were incubated without any additions (A and D), with 100 μM bbG (B), and with 10 mM CD (C) for 1 h at 37°C, respectively. Cells were fixed and labeled with anti-GH antibody followed by Cy3-conjugated anti-sheep IgG (A–C) or subjected to antibody-induced cross-linking before fixation (D) as described in MATERIALS AND METHODS.