Figure 2.

Mitotic oscillations induced by bimA1^APC3 upon rapid temperature shift. (A) CMI of a bimA1^APC3 mutant strain after rapid temperature shift. (B) Autoradiograms of p34^cdc2 H1 and NIMA kinase activities and Western blot analysis of NIMA, NIME^cyclinB, and p34^cdc2. (C) Nuclear division and germling growth of the nimA5 + bimA1^APC3 mutant cells after rapid temperature upshift. Spores were first germinated at 32°C for 7.5 h, and, after harvesting by centrifugation, the germlings were rapidly transferred to new medium prewarmed to 42°C and incubated at 42°C for 8 h. The number of nuclei and germling length were measured before and 8 h after temperature shift.