Skip to main content
. 1999 Oct;10(10):3223–3238. doi: 10.1091/mbc.10.10.3223

Figure 4.

Figure 4

Rad3p dominant negative alleles disrupt the checkpoint coupling mitosis to completion of DNA replication. (A) Percent aberrant mitoses after treatment with 10 mM HU of wild-type cells (TE235) expressing rad3+ (pTE157), rad3-C725 (pTE446), rad3-KD (pTE791), rad3-N775 (pTE696), and rep3x (pTE101). The results shown are the average of two independent experiments. (B) Photomicrographs of cells cultured in 10 mM HU for 10 h and then fixed and stained with the DNA-specific dye DAPI. Cells were wild-type (TE235) expressing rad3-N775, rad3-KD, rad3-C725, and rad3+. Aberrant mitoses are indicated with arrows. (C) FACS analysis of cells incubated with HU for indicated times (at left). Wild-type cells expressing rep3x control (vector), rad3-N775 (N775), rad3+, and rad3-KD are shown, as well as rad3Δ cells expressing the rep3x vector (rad3Δ). Note that overexpression of rad3+ or rad3-KD inhibits DNA replication after exposure to HU. See text for more details.