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. 1999 Oct;10(10):3223–3238. doi: 10.1091/mbc.10.10.3223

Figure 7.

Figure 7

Leucine zipper and P-site are required for normal function of Rad3 protein. (A) Rad3 proteins are diagrammed approximately to scale. See Figure 5 for explanation of symbols. (B) rad3Δ (TE890) cells transformed with rep3x (pTE101), rep1-HA-rad3+ (pTE541), rep1-HA-rad3-LZ (pTE716), or rep1-HA-rad3-P (pTE706) were grown on plates containing 10 mM HU. (C) Relative viability of the same strains after exposure to the indicated doses of UV irradiation. Results shown are the average of two independent experiments. (D) Rad3-LZp and Rad3-Pp are active kinases. Anti HA-immunopreciptates were prepared from rad3Δ (TE890) cells transformed with the plasmids indicated above. Half of each immunoprecipitate was assayed for PHAS-1 kinase activity, and the other half was subjected to SDS-PAGE, Western blotted, and probed with HA monoclonal antibody.