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. 1999 Oct;10(10):3223–3238. doi: 10.1091/mbc.10.10.3223

Figure 8.

Figure 8

Rad3p leucine zipper motif is not required for self-association or for interaction with Chk1p. Wild-type cells (TE236) were cotransformed with the indicated rad3 and/or chk1 alleles, and myc immunoprecipitations were performed on extracts from the indicated strains. Samples were split, resolved on SDS-PAGE gels, blotted, and probed with either anti-HA antibodies (top) or anti-myc antibodies (bottom). (A) Self-association assay. Lane 1, rep41-HA-rad3+ (pTE521) and rep42-myc-rad3+ (pTE748); lane 2, rep41-HA-rad3-LZ (pTE746) and rep42-myc-rad3-LZ (pTE747); lane 3, rep41-HA-rad3-LZ and rep42-myc-rad3+; lane 4, rep41-HA-rad3+ and rep42-myc-rad3-LZ; lane 5, rep41-HAtag vector (control) (pTE119) and rep42-myc-rad3+; lane 6, rep41-HA-rad3+ and rep42-myctag vector (control) (pTE120). (B) Chk1p/Rad3p coimmunoprecipitation assay. Lane 1, rep41-HA-rad3+ and rep42-myc-chk1+ (pTE792); lane 2, rep41-HA-rad3-LZ and rep42-myc-chk1+; lane 3, rep41-HA-rad3+and rep42-myctag vector (control); lane 4, rep41-HAtag vector and rep42-myc-chk1+.