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. 1999 Oct;10(10):3239–3250. doi: 10.1091/mbc.10.10.3239

Figure 2.

Figure 2

Rab5-induced lamellipodia formation is PI3-K– and Rac-independent. Shown is immunofluorescence microscopy of the actin cytoskeleton of A14 cells (A) transfected with either L79-Rab5 or V12-Ras or stimulated with insulin (as indicated per row) and (co)transfected with control plasmid or N17-Rac or treated with LY 294002 (as indicated per column), or (B) cotransfected with V12-Ras and PAK-RBD or L79-Rab5 and PAK-RBD, as indicated. Arrowheads indicate the Cy3-positive cells stained for the expression of L79-Rab5, V12-Ras, or in case of their cotransfection with L79-Rab5, N17-Rac, or PAK-RBD. Only the actin staining as detected by phalloidin-FITC is shown. Bar, 30 μm. The scale bar in the top left picture applies to all others, unless a different size scale bar is shown.