Rab5-induced lamellipodia formation is Ras-, Cdc42-, and Rho-independent. Immunofluorescence microscopy of the actin cytoskeleton of A14 cells cotransfected with L79-Rab5 and N17-Ras, N17-Cdc42, or N19-Rho is shown. Arrowheads indicate the Cy3-positive cells stained for expression of the dominant negative GTPases. Only the actin staining as detected by phalloidin-FITC is shown. Bar, 30 μm.