Figure 4.

Insulin-, Ras-, and Rac-induced lamellipodia formation is Rab5-independent. Shown is immunofluorescence microscopy of the actin cytoskeleton of A14 cells transfected with V12-Rac alone, or cotransfected with V12-Rac, V12-Ras, V12-Cdc42, or V14-Rho and N34-Rab5, or transfected with N34-Rab5 alone and stimulated for 5 min with insulin (as indicated). Arrowheads indicate the Cy3-positive cells stained for expression of either V12-Rac or, in the case of cotransfection, N34-Rab5. Only the actin staining as detected by phalloidin-FITC is shown. Bar, 30 μm.