Figure 5.

Rab5 induces cell migration that is actin polymerization-dependent but microtubule-independent. Shown is time-lapse video microscopy of the migration of A14 cells that were (A) transfected with L79-Rab5 (please note that the cell only starts to migrate after polarizing its lamellipodium), (B) L79-Rab5–transfected and treated at t = 58 min with either 1 μM cytochalasin D (please note that only the cell with the polarized lamellipodium in the bottom right corner shows migration between 0 and 20 min, until its lamellipodium depolarizes), and (C) L79-Rab5–transfected and treated at t = 0 min with 10 μg/ml nocodazole. The inset (D) shows the actin cytoskeleton and confirms depolymerization of microtubules (compare with Figure 1) of cells, including a cell displaying a lamellipodium as a consequence of L79-Rab5 transfection, after treatment with 10 μg/ml nocodazole for 30 min. Bar, 30 μm.