Figure 2.

Northern blot detection of pre-tRNA^Ile_UAU, mature tRNA^Ile_UAU, and mature tRNA^Ile_AAU. tRNAs were isolated from EE1b-35 (lanes 1, 2, 5, 6, 9, and 10) and EE1b-6 (lanes 3, 4, 7, 8, 11, and 12) cells grown either at 23°C (lanes 1, 3, 5, 7, 9, and 11) or shifted to 37°C for 1 h (lanes 2, 4, 6, 8, 10, and 12). Lanes 1–4 were incubated with probe 04, lanes 5–8 were incubated with probe 05, and lanes 9–11 were incubated first with probe 05 and then stripped and reprobed with probe 03. In lanes 1–4, the top band corresponds to 5′ and 3′ extended intron-containing pre-tRNA species, the next band corresponds to end-matured intron-containing pre-tRNA, and the bottom band corresponds to mature tRNA^Ile_UAU.