Figure 4.

Identification of tRNA transport mutants is possible using fluorescence in situ hybridization. Parent W303 and nup116Δ strain SWY27 were grown at 23°C, and log phase cells were shifted to 37°C for 1 h. (A) In situ hybridization detection of Poly(A) RNA with digoxigenin-labeled probe 02 in W303 cells. (C) Detection of Poly(A) RNA with digoxigenin-labeled probe 02 in SWY27 cells. (E) Detection of mature tRNA^Ile_AAU with digoxigenin-labeled probe 05 in W303 cells. (G) Mature tRNA^Ile_AAU detection with digoxigenin-labeled probe 05 in SWY27 cells. (B, D, F, and H) DAPI staining of cells shown in A, C, E, and G, respectively.