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. 1998 Nov;9(11):3041–3055. doi: 10.1091/mbc.9.11.3041

Figure 7.

Figure 7

Mutation in RNA1 causes accumulation of mature tRNA in the nucleus. The same cultures of parent EE1b-35 and rna1-1 mutant strain EE1b-6 that were grown at 23°C and used for Figure 3 were shifted to 37°C for 3 h. (A) Detection of Poly(A) RNA with probe 02 in EE1b-35 cells. (C) Detection of Poly(A) RNA with probe 02 in EE1b-6 cells. (E) Detection of mature tRNAIleAAU with probe 05 in EE1b-35 cells. (G) Detection of mature tRNAIleAAU with probe 05 in EE1b-6 cells. (B, D, F, and H) DAPI staining of cells shown in A, C, E, and G, respectively. Arrows point to cells displaying nuclear accumulations.