Figure 3.

p84N5 contains a death domain. (A) Top of figure indicates sequence identities (shaded boxes) between helix 4 and 5 of the death domains from the indicated proteins. The bar underneath the sequence identifies the extent of the deletion in N5Δα4. The asterisks indicate the positions of point mutations in N5-PP and N5-R. The numbers indicate the corresponding p84N5 amino acids. SAOS-2 or 293 cells were transfected with the indicated plasmids, and clonogenicity of transfected cells was determined as in Figure 1C. The results are expressed as the mean of at least three experiments. (B) 293 cells were transfected with pCMV (lane 1), pCMVN5 (lane 2), pCMVN5Δα4 (lane 3), pCMVN5-PP (lane 4), and pCMVN5-R (lane 5). Extracts were prepared from transfected cells, and expression of p84N5 protein were analyzed by Western blotting with anti-N5 monoclonal antibody 5E10 as indicated in MATERIALS AND METHODS.