Figure 7.

Two-hybrid interaction of Cdc4p with wild-type and mutant Cdc6p. Strains were prepared as described in MATERIALS AND METHODS. All strains carry a plasmid bearing a truncated allele of CDC4 fused to the activation domain of GAL4 (Drury et al., 1997). Strains also carry BTM116-based plasmids, which bear the lexA DNA binding domain fused to wild-type and mutant alleles of CDC6, as indicated in the schematic. β-Galactosidase reactions were carried out as described in MATERIALS AND METHODS.