Figure 9.

Overexpression of cdc6-Fp in a cdc16 mutant that rereplicates its DNA is lethal. The respective phosphorylation site cdc6-A, -B, -C, -D, -E, or -F mutant genes were introduced into strain JLC1402. The transformants were streaked on YPD (left) or YPRG (right) plates to induce the cdc6 mutant proteins. Plates were incubated at 30°C for 4 d. In the course of carrying out these studies we discovered that JLC1402 is a diploid, cdc16-264/+ heterozygote and not a cdc16-264 haploid as originally thought. All experiments using this mutant were repeated in the appropriate haploid, and the same results were obtained.