Table 3.

Plasmids for in vitro ubiquitination and stability studies

Sites mutated	Bluescript construct	GAL plasmid
Wild type	pELS91	pELS147
Δ8–46	pELS144	pELS148
Δ3–16	pELS92	pELS149
Δ13–26	pELS93	pELS150
Δ23–36	pELS94	pELS151
Δ33–46	pELS95	pELS152
NLS	pELS105	pELS156
A	pELS164	pELS176
B	pELS193	pELS193
C	pELS177	pELS177
D	pELS165	pELS178
E	pELS166	pELS179
F	pELS167	pELS194
A + B	pELS187	pELS198
B + C	pELS162	pELS180
A + C	pELS197	pELS199
A–C	pELS188	pELS200
E + F	pELS163	pELS181
A–F	pELS159X	pELS175