Table 2.
Yeast strains
| Strain | Genotypea | Source |
|---|---|---|
| HH1-KAT6 | MATα ade2-1 can1-100 his3-12,16 leu2-3,112 trp1-1 ura3-1 Δhsc82::LEU2 Δhsp82::LEU2 / GAL1-hHSP90β-CEN/ARS-HIS3 [pGAL1-hhsp90] | S. Lindquist (described in Palmer et al., 1995) |
| HH1ab | MATa ade2-1 can1-100 his3-12,16 leu2-3,112 trp1-1 ura3-1 Δhsc82::LEU2 Δhsp82::LEU2 | This article |
| HH1a-pHCA/Hsp82wt | HH1a / HSP82-CEN/ARS-HIS3 [pHCA/Hsp82] | This article |
| DP120 | HH1a / HSP82-2μ-URA3 [p2U/Hsp82] | This article |
| DP121 | DP120 / fus1::HIS3 | This article |
| DP122 | HH1a / fus1::HIS3 / HSP82-CEN/ARS-TRP1 [pTCA/Hsp82] | This article |
| DP123 | HH1a / fus1::HIS3 / HSP82 G313N-CEN/ARS-TRP1 [pTCA/Hsp82 G313N] | This article |
| DP124 | HH1a / fus1::HIS3 / hHSP90β-2μ-TRP1 [p2TG/hHsp90β] | This article |
| HH1a-p2G/Hsp82wt | HH1a / HSP82-2μ [p2G/Hsp82] | This article |
| HH1a-p2G/Hsp82 G313N | HH1a / HSP82 G313N-2μ [p2G/Hsp82 G313N] | This article |
| HH1a-p2G/hHsp90β | HH1a / hHSP90β-2μ [p2G/hHsp90β] | This article |
| HH1a-p2TG/flag.Hsp82wt | HH1a / flag.Hsp82-2μ-TRP1 [p2TG/flag.Hsp82wt] | This article |
| JC6ab | MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 Δhsc82::LEU2 Δhsp82::LEU2 Δpep4::HIS3 | S. Lindquist |
| JC6a-Hsp82 | JC6a / HSP82-CEN/ARS-TRP1 [pTCA/Hsp82] | This article |
| JC6a-Hsp82 G313N | JC6a / HSP82 G313N-CEN/ARS-TRP1 [pTCA/Hsp82 G313N] | This article |
| JC6a-hHsp90β | JC6a / hHSP90β-2μ-TRP1 [p2TG/hHsp90β] | This article |
| RMY326 | MATa his3 leu2-3,112 trp1-1 ura3-52 | R. Movva |
| E929-6C-20 | E929-6C-0 ste11-Δ6 | Rhodes et al., 1990 |
a
Episomes are indicated after a slash with the name of the plasmid in brackets.
b
HH1a and JC6a represent related genotypic backgrounds of many strains rather than real strains (they are not viable without Hsp90 function being provided, for example, by a plasmid).