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Effect of SIBA on the accumulation of cyclin B1. Thirty prophase oocytes, microinjected or not with p21cip1, were cultured with or without of 0.75 mM SIBA for 2 h. Then, progesterone (Pg) was added or not and oocytes were cultured overnight. Oocytes were collected, homogenized, and analyzed by immunoblotting for Mos (A) or cyclin B1 (B). An amount equivalent to three oocytes was loaded on each lane.
