Figure 3.

Expression of truncated minigene deletion constructs. (A) Diagrams depicting deletion constructs. Thin lines indicate untranscribed flanking regions, thick lines indicate introns, and boxes indicate exons (open boxes represent UTRs and shaded boxes translated regions of the exons). A truncated IC70 gene was created by removing two BglII fragments from 6 kb genomic clone pB70S6. 5′- And 3′-deletions were then created by unidirectional exonuclease III digestion, and internal deletions were obtained by ligating together selected 5′- and 3′-deletions. (B) Expression of truncated gene T-416. RNA isolated before deflagellation (ND) and at indicated times after deflagellation was probed with an IC70 cDNA. The truncated gene expressed a smaller mRNA (1.6 kb) distinguishable from that of endogenous IC70 mRNA (2.6 kb). (C) The blot from panel B was stripped and reprobed with CBLP to confirm that equal amounts of mRNA were loaded. (D) Northern blots of RNA isolated before deflagellation (ND) and 30 min after deflagellation (DF) from cells transformed with the constructs shown in panel A. Blots were hybridized separately (T-96, T-45) or simultaneously (I-52–32, I-37–13) with IC70 and CBLP probes.