Figure 3.

Sequence of the darA gene. The open reading frame of the darA gene is divided into four exons (bold capital letters) that extend over 2301 bp and encode a protein of 766 amino acids with a predicted molecular mass of 87.7 kDa. The darA coding region is interrupted by three introns (underlined bold lowercase letters) of 110, 100, and 144 bp. The 5′ and 3′ flanking sequences are shown in lower case letters. The 5′ flanking portion of the gene has two GC-rich clusters (boxed letters) that are often associated with promoter elements in Dictyostelium (Driscoll et al., 1988). After the stop codon at the 3′ end of the gene, there are four poly(A) signals (AATAAA) immediately preceding the site of polyadenylation at residue 3158. The predicted amino acid sequence is shown below the coding sequence. The three peptides that were derived from microsequencing are shown in white letters on a black background. The amino acid sequence is numbered on the left and the nucleic acid sequence on the right. This sequence has the GenBank accession number AF006055.