Figure 5.

Darlin is similar to mammalian smgGDS and has 11 armadillo-like repeats. (A) Diagram indicating the position of armadillo-like repeats in darlin and smgGDS. Each rectangle represents a single repeat. Almost the entire sequence of human smgGDS protein consists of 11 armadillo repeats. The Dictyostelium (D.d.) darlin protein also has 11 armadillo-like repeats. The shaded regions (I–II) indicate regions of homology between the two proteins as shown in B. (B) The darlin sequence was compared with the bovine and human smgGDS sequences from the GenBank database using BlastP. Residues that are identical between the darlin protein and either mammalian smgGDS protein are shown in white letters on a black background. Residues that represent conservative substitutions are shown in black letters on a gray background. The amino acid sequence is numbered on both sides of these regions of similarity.