Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1987 Nov;61(11):3485–3490. doi: 10.1128/jvi.61.11.3485-3490.1987

In vitro immortalization of marmoset cells with three subgroups of herpesvirus saimiri.

E Szomolanyi 1, P Medveczky 1, C Mulder 1
PMCID: PMC255946  PMID: 2822956

Abstract

Sequences within the rightmost 7 kilobases of the unique L DNA of herpesvirus saimiri are required for oncogenicity of the virus. The same DNA region has been found to be highly variable among different strains of herpesvirus saimiri. On the basis of this variability, herpesvirus saimiri strains were classified into groups A, B, and non-A, non-B. Herpesvirus saimiri strains representing the three groups were used successfully for in vitro immortalization of phytohemagglutinin-activated, interleukin 2 (IL-2)-expanded peripheral blood lymphocytes of common marmosets (Callithrix jacchus). Peripheral blood leukocytes could be immortalized from only a subset of common marmosets (5 of 13). All of the immortalized cell lines contained covalently closed circular viral DNA molecules and initially showed a low level of virus production. Cells immortalized by group A and group non-A, non-B strains did not require IL-2 in the medium. However, the only group B immortalized cell line, 473-SMHI, did not grow well in the absence of IL-2. The different characteristics of cell lines immortalized by herpesvirus saimiri strains belonging to different groups may help to elucidate some functions coded by the highly variable DNA region which is involved in the oncogenic process.

Full text

PDF
3488

Images in this article

3488Image
on p.3488

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ablashi D. V., Schirm S., Fleckenstein B., Faggioni A., Dahlberg J., Rabin H., Loeb W., Armstrong G., Peng J. W., Aulahk G. Herpesvirus saimiri-induced lymphoblastoid rabbit cell line: growth characteristics, virus persistence, and oncogenic properties. J Virol. 1985 Sep;55(3):623–633. doi: 10.1128/jvi.55.3.623-633.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bankier A. T., Dietrich W., Baer R., Barrell B. G., Colbère-Garapin F., Fleckenstein B., Bodemer W. Terminal repetitive sequences in herpesvirus saimiri virion DNA. J Virol. 1985 Jul;55(1):133–139. doi: 10.1128/jvi.55.1.133-139.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Colbere-Garapin F., Chousterman S., Horodniceanu F., Kourilsky P., Garapin A. C. Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3755–3759. doi: 10.1073/pnas.76.8.3755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daniel M. D., Meléndez L. V., Hunt R. D., King N. W., Anver M., Fraser C. E., Barahona H., Baggs R. B. Herpesvirus saimiri: VII. Induction of malignant lymphoma in New Zealand white rabbits. J Natl Cancer Inst. 1974 Dec;53(6):1803–1807. [PubMed] [Google Scholar]
  5. Desrosiers R. C., Burghoff R. L., Bakker A., Kamine J. Construction of replication-competent Herpesvirus saimiri deletion mutants. J Virol. 1984 Feb;49(2):343–348. doi: 10.1128/jvi.49.2.343-348.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Desrosiers R. C., Falk L. A. Herpesvirus saimiri strain variability. J Virol. 1982 Jul;43(1):352–356. doi: 10.1128/jvi.43.1.352-356.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Desrosiers R. C., Silva D. P., Waldron L. M., Letvin N. L. Nononcogenic deletion mutants of herpesvirus saimiri are defective for in vitro immortalization. J Virol. 1986 Feb;57(2):701–705. doi: 10.1128/jvi.57.2.701-705.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gardella T., Medveczky P., Sairenji T., Mulder C. Detection of circular and linear herpesvirus DNA molecules in mammalian cells by gel electrophoresis. J Virol. 1984 Apr;50(1):248–254. doi: 10.1128/jvi.50.1.248-254.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaschka-Dierich C., Werner F. J., Bauer I., Fleckenstein B. Structure of nonintegrated, circular Herpesvirus saimiri and Herpesvirus ateles genomes in tumor cell lines and in vitro-transformed cells. J Virol. 1982 Oct;44(1):295–310. doi: 10.1128/jvi.44.1.295-310.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koomey J. M., Mulder C., Burghoff R. L., Fleckenstein B., Desrosiers R. C. Deletion of DNA sequence in a nononcogenic variant of Herpesvirus saimiri. J Virol. 1984 May;50(2):662–665. doi: 10.1128/jvi.50.2.662-665.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Laufs R., Fleckenstein B. Susceptibility to Herpesvirus saimiri and antibody development in old and new world monkeys. Med Microbiol Immunol. 1973 Mar 8;158(3):227–236. doi: 10.1007/BF02120558. [DOI] [PubMed] [Google Scholar]
  12. Laufs R., Steinke H., Steinke G., Petzold D. Latent infection and malignant lymphoma in marmosets (Callithrix jacchus) after infection with two oncogenic herpesviruses from primates. J Natl Cancer Inst. 1974 Jul;53(1):195–199. doi: 10.1093/jnci/53.1.195. [DOI] [PubMed] [Google Scholar]
  13. Medveczky P., Szomolanyi E., Desrosiers R. C., Mulder C. Classification of herpesvirus saimiri into three groups based on extreme variation in a DNA region required for oncogenicity. J Virol. 1984 Dec;52(3):938–944. doi: 10.1128/jvi.52.3.938-944.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murthy S., Kamine J., Desrosiers R. C. Viral-encoded small RNAs in herpes virus saimiri induced tumors. EMBO J. 1986 Jul;5(7):1625–1632. doi: 10.1002/j.1460-2075.1986.tb04405.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rabin H., Hopkins R. F., 3rd, Desrosiers R. C., Ortaldo J. R., Djeu J. Y., Neubauer R. H. Transformation of owl monkey T cells in vitro with Herpesvirus saimiri. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4563–4567. doi: 10.1073/pnas.81.14.4563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rangan S. R., Martin L. N., Enright F. M., Allen W. P. Herpesvirus saimiri-induced malignant lymphoma in rabbits. J Natl Cancer Inst. 1976 Jul;57(1):151–156. doi: 10.1093/jnci/57.1.151. [DOI] [PubMed] [Google Scholar]
  17. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  18. Schirm S., Müller I., Desrosiers R. C., Fleckenstein B. Herpesvirus saimiri DNA in a lymphoid cell line established by in vitro transformation. J Virol. 1984 Mar;49(3):938–946. doi: 10.1128/jvi.49.3.938-946.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wright J., Falk L. A., Wolfe L. G., Ogden J., Deinhardt F. Susceptibility of common marmosets (Callithrix jacchus) to oncogenic and attenuated strains of Herpesvirus saimiri. J Natl Cancer Inst. 1977 Nov;59(5):1475–1478. [PubMed] [Google Scholar]
  20. Youssoufian H., Hammer S. M., Hirsch M. S., Mulder C. Methylation of the viral genome in an in vitro model of herpes simplex virus latency. Proc Natl Acad Sci U S A. 1982 Apr;79(7):2207–2210. doi: 10.1073/pnas.79.7.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES