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. 1999 Oct;10(10):3301–3316. doi: 10.1091/mbc.10.10.3301

Figure 6.

Figure 6

Overexpression of the Clb1 and Clb2 mitotic cyclins abrogates filamentous development and antagonizes RAS/STE signaling. Strains containing integrated copies of the CLB1 and CLB2 genes under an inducible GAL1,10 promoter and a control plasmid or the STE11-4 or RAS2-Val19 allele were grown on nitrogen starvation (SLAD) or synthetic low-ammonia galactose (SLARG) media for 24 h. Both basal and enhanced filamentous growth are abolished by Clb1 or Clb2 overexpression on SLARG media, indicating that the RAS/STE pathway may function upstream of Clb1 and Clb2. Representative colonies of each strain were photographed after 24 h at 22°C. Bars, 50 μm.