Figure 2.

Time course of JNK1, JNK2, Akt, Ras, Bcl-2, Bcl-x, and Bax expression in IL-4-deprived cells. (A) Control or IL-4-deprived cells (2 × 10⁶) were harvested at different time points, lysed, and protein extracts separated in SDS-PAGE. Proteins were transferred to nitrocellulose and probed sequentially with anti-JNK1, -JNK2, -Akt, and -Ras antibody, the last as an internal protein loading control. Protein bands were detected using the ECL system. Similar results were obtained in three independent experiments. Molecular mass markers are indicated. Densitometric analysis of JNK2 expression is shown below the blot. (B) TS1αβ cells were stimulated by IL-4 or deprived of lymphokine. At different time points, cells (2 × 10⁶) were lysed and protein extracts separated, transferred to nitrocellulose, and probed with anti-Bcl-2, -Bax, and -Bcl-x. Cells maintained in IL-2 were used as a positive control. Protein bands were detected using the ECL system. An internal control of homogeneous Ras expression in all samples is shown. Similar results were obtained in three independent experiments.