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Gel filtration analysis of the Sec34p/Sec35p complex. Lysates prepared from wild type (A) and the sec34-1 mutant (B) were chromatographed on a Superose 6 column. Wild-type fractions containing Sec34p and Sec35p (A) and mutant fractions containing Sec35p (B) were detected by Western blot analysis. The positions of the markers used to calibrate the column are shown. In B, Sec35p was not detected in any fractions other than those shown.
