Figure 2.

Effect of the N-cadherin stimulation on myotube formation in C2, L8, and L84 myogenic cell lines. C2, L8, and L84 myoblasts were treated with NEC and anti-N-cadherin (anti N-cad) BE beads for the indicated periods, fixed, and stained with Giemsa. C2 cells were maintained in growth medium, whereas for L8 and L84 cells the culture medium was replaced with differentiation medium simultaneously with the addition of beads. Media were changed every second day. Notice that the number of myotubes in the field is higher for the cells treated with cadherin-reactive beads. Arrows point to some of the multinucleated myotubes. Bar, 100 μm.