Figure 6.

Inhibition of S-phase entry in C2 myoblasts treated with N-cadherin–reactive beads. C2 myoblasts were plated and cultured overnight on gelatin-coated coverslips at 50% confluence. Cells were incubated with the various beads for 24 or 48 h and pulsed for 45 min with BrdU, fixed, and stained with anti-BrdU antibodies. Nuclei were visualized by DAPI, and the percentage of BrdU-positive nuclei was calculated. Each column represents the mean ± SD of 3 independent experiments; 1500 cells were counted in each case. Analysis of the differences between the cells treated with anti-N-cadherin and control beads was examined using Student’s t test (one tailed, paired) pointed to a significant decrease in the percentage of cells in S phase after 24 h of treatment (p = 0.002) and especially after 48 h of treatment (p = 0.0017).