Table 2.
PCR primers and conditions for quantitative PCR
| Gene | Forward and reverse primer (5' – 3') | Product size (bp) | Tm (°C) |
| Real-time PCR | |||
| Control gene BCAL1861 (phaC) | AGACGGCTTCAAGGTGGT ACACGGTGTTGACCGTCA |
470 | 62/66 |
| BCAL0270 | GCGCGAACCCGATCGAATTC GCCCGCATCCACCAGAAGTG |
400 | 66 |
| BCAM2753 | GAACCGCAGACGCTGTACTC AAACGCTCGGTGTTGCGGAC |
380 | 62 |
| BCAL1107 | GAATCGACGTATCGGCTCGT ATGATGTGCTTCGGGTTCTTG |
377 | 66 |
| BCAM1676 | GACGACCTGTTCCTGCTGTG CGGCTCTTCCTGATGACGTG |
534 | 62 |
| Semi-quantitative PCR | |||
| BCAL1165 | TGACGCTCGGCACCGTTGAC GCGTGGACCTGCTCGATCTC |
400 | 66 |