Table 3.
Confirmation of B. cenocepacia gene expression by Real Time PCR
| Real-time PCR fold change using delta delta CT method | ||||
|
Gene expression determined from (infection; clinical condition): |
BCAL0270 (ferric reductase transmembrane component) |
BCAM2753 (ohr gene, organic hydroperoxide resistance protein) |
BCAL1107 (oxidoreductase) |
BCAM1676 (nitrite/sulfite reductase) |
|
Microarray dataset (Sputum 1, 2, and 3; see below) |
↑ 36 | ↑ 8 | ↑ 66 | ↑ 20 |
|
Sputum 2 (B. cenocepacia; stable) |
↑ 160 | ↑ 100 | ↑ 914 | ↑ 97 |
|
Sputum 3 (B. cenocepacia; exacerbation) |
↑ 65 | ↑ 40 | ↑ 299 | ↑ 4 |
|
Sputum 4 (B. cenocepacia; stable) |
↑ 25 | ↑ 22 | ↑ 235 | - |
|
Sputum 5 (P. aeruginosa; exacerbation) |
↑ 22 | ↑ 71 | ↑ 189 | - |
|
Sputum 6 (P. aeruginosa; stable) |
↑ 3 | ↑ 3 | ↑ 12 | ↑ 94 |
| Average for sputum growth | ↑ 55 | ↑ 47 | ↑ 330 | ↑ 65 |
| Fold change in minimal media with: | ||||
| No FeSO4 | ↑ 2 | - | ||
| 0.1% H2O2 | - | ↑ 5 | ||
| 0.15% H2O2 | - | ↑ 3 | ||
| 0.002% t-butyl hydroperoxide | - | ↑ 2 | ||