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. Author manuscript; available in PMC: 2009 Sep 5.
Published in final edited form as: Cell. 2008 Sep 5;134(5):782–792. doi: 10.1016/j.cell.2008.06.036

Figure 3. HIV Envelope-CXCR4 Signaling Promotes Cortical Actin Dynamics.

Figure 3

(A, B) Actin changes mediated by gp120 or SDF-1. Cells were treated with gp120IIIB or SDF-1, fixed, permeabilized, and then stained with FITC-phalloidin for flow cytometry. (C, D) HIV-mediated actin depolymerization was dependent on PTX-sensitive Gαi signaling. Cells were not treated (C) or treated with PTX (D) for 2 hours, infected, and then stained with FITC-phalloidin. (E, F) Confocal microscopy of FITC-phalloidin staining of cells treated with gp120IIIB, SDF-1, HIV or PTX plus HIV. The full time courses of these treatments are shown in Figures S12 to S14. Red arrows indicate localized actin polymerization. (G) Actin depolymerization triggered by stimulation of CXCR4. Cells were stimulated with either anti-CD4 or anti-CXCR4-IMag particles, and stained with FITC-phalloidin.