. 2008 Oct 10;3(10):e3366. doi: 10.1371/journal.pone.0003366

Table 1. PCR primers and conditions.

	Nest 1		Nest 2
Pvama1 1663 bp	Pvama1_F16	5′ gcg gtt act tcc acc cc 3′	Pvama1_F29	5′ gca aac caa atc gct gcc 3′
	Pvama1_R613	5′ gcg tgg tgt ggg agg ccc 3′	Pvama1_R598	5′ gcc tcg ggg tcg agc atc tcg tc 3′
	Nest 1 PCR:	94°C^{3 min}×1 cycle, (94°C^{1 min}−46°C^{1 min}−72°C^{2.5 min})×40 cycles, 72°C^{10 min}×1	Nest 2 PCR:	94°C^{3 min}×1 cycle, (94°C^{1 min}−57°C^{1 min}–72°C^{2.5 min})×40 cycles, 72°C^{10 min}×1
	Fwd sequencing:		Rev sequencing
	Pvama1_F142	aga att cca gct gga aga tg	Pvama1_R138	ttc cac ttc tgc atc ttc ccc
	Pvama1_F301	cgt aaa aat tta gga aac gcc	Pvama1_R293	aca ttt ttg ctc aaa tac acc
	Pvama1_F441	ccc ctg cag cat ata taa aga c	Pvama1_R442	ggg gaa atc ccg gtc tac ttc
Pfama1 1770 bp	Pfama1_F5^*	5′ tgc gta tta tta ttg agc 3′	Pfama1_F10^*	5′ gag cgc ctt tga gtt tac 3′
	Pfama1_R613^*	5′ gtg ttg tat gtg atg ctc 3′	Pfama1_R598^*	5′ gcc tca gga tct aac att tca tc 3′
	Nest 1 PCR:	94°C^{3 min}×1 cycle, (94°C^{1 min}−46°C^{1 min}−72°C^{2.5 min})×40 cycles, 72°C^{10 min}×1	Nest 2 PCR:	94°C^{3 min}×1 cycle, (94°C^{1 min}−60°C^{1 min}−72°C^{2.5 min})×40 cycles, 72°C^{10 min}×1
	Fwd sequencing:		Rev sequencing:
	Pfama1_F143	gac ttc cat cag gga aat gtc c	Pfama1_R188	gag gtt ctg ttg gag gaa aag c
	Pfama1_F312	cgg att atg ggt cga tgg aaa ttg	Pfama1_R350	tta ggt tga tcc gaa gca ctc aa
	Pfama1_F485	gac agt tta aaa tgc cca tgt gc	Pfama1_R492	cac atg ggc att tta aac tgt c

(Polley & Conway, 2001).