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. 2008 Oct 1;22(19):2633–2638. doi: 10.1101/gad.1674108

Figure 2.

Figure 2.

HBO1 association with origins is restricted to G1. (A) Expression level of HBO1, ORC1, Cdt1, and MCM2 mRNAs in CCL-156 cells in culture (asynchronously), following serum deprivation and cell cycle exit (G0), and following readdition of 20% serum in the media for 6 h after serum deprivation (G0 + serum). The left panel illustrates the cell cycle profile of samples used in the experiment. “no RT” referrs to the control condition where cDNA synthesis was omitted prior to PCR analysis. (B) Expression level of HBO1, MCM2, and ORC2 proteins upon readdition of serum for indicated number of hours. (C) ChIP analysis of HBO1 binding at origins and control sites in growing and serum-starved cells. (D) ChIP analysis of HBO1 binding at origins in cells staged at different phase of the cycle.