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. 2008 Oct 1;22(19):2633–2638. doi: 10.1101/gad.1674108

Figure 4.

Figure 4.

Cdt1 directly interacts with HBO1. (A) Detection of HBO1 association with Cdt1 by reverse coimmunoprecipitation in HeLa cells overexpressing either HA-Cdt1 or Flag-HBO1. (B) Same experiment using antibodies directed against endogenous HBO1 and Cdt1. (C) Interaction of in vitro translated Flag-HBO1 with full-length His-Cdt1 and His-Geminin. (D) Interaction of purified in vitro translated Flag-HBO1 with His-Cdt1. (E) In vitro preformed Cdt1/HBO1 complex were challenged by increasing amount of bacterially produced Geminin. (F) Mix of Cdt1, Geminin, and HBO1 proteins were incubated with the indicated antibodies, and the purified complexes was determined by Western blot analysis. (G) Role of the MYST zinc finger. (Left panel) The indicated HBO1 derivatives were analyzed for interaction with Cdt1 or occupancy at the MCM4 and LaminB2 origins. (*) P < 0.05 ; (**) P < 0.15.