Figure 1.
CDK inhibition does not prevent JM resolution or SC breakdown. NDT80-IN cdc28-as1 (MJL3232) cells were sporulated for 6 h, and the culture was divided into three portions: uninduced (−ED; no ED or 1NMPP1; NDT80off ; blue line), induced (+ED; 1 μM β-estradiol added at 7 h; NDT80on; red line), and CDK-inhibited (+1NMPP1 + ED; 20 μM 1-NM-PP1 added at 6 h followed by 1 μM ED at 7 h; CDKoff; NDT80on; green line). Arrows indicate times of addition. See Supplemental Figure 4 for details of digests. (A) JM recombination intermediates. Blots containing XmnI digests were probed with ARG4 sequences. (P1, P2) Parental fragments containing inserts at LEU2 and HIS4, respectively. Frequencies of JMs are plotted in C as a percentage of total lane signal. (B) CO and NCO products. Blots containing EcoRI/XhoI digests were probed with HIS4 sequences. Frequencies of CO and NCO products are plotted in D and F, respectively, as a percentage of total lane signal. (E) Fraction of cells completing the first meiotic nuclear division. (G,H) Western blots detecting Zip1 (G) and Cdc5 (H). Graphs show normalized Zip1 and Cdc5 levels, which were determined as described in Material and Methods.