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. 2008 Oct 1;22(19):2645–2650. doi: 10.1101/gad.1711308

Figure 5.

Figure 5.

Sumf1 regulates FGF18 activity during endochondral ossification. (A) H/E staining of femurs showing shortening of Sumf1−/− bone length (arrowheads) and its rescue in Sumf1−/−;fgf18+/− mice. Brdu staining (B) and in situ expression of α1(II) Collagen (C) and α1(X) Collagen (D) in wild-type (left), Sumf1−/− (middle), and Sumf1−/−;fgf18+/− mice. (E) Quantification of femoral length, BrdU index, and cell number in newborn wild-type, Sumf1−/−, and Sumf1−/−;fgf18+/− mice. At least three mice were analyzed per each genotype. Error bars represent SEM. Student’s test (*) P < 0.05; (**) P < 0.01. (F) Primary chondrocytes from wild-type and Sumf1−/− mice treated with Fgf18 (20 ng/mL) for the indicated period of time. Note the more sustained phosophorylation of ERK and 70S6k in Sumf1−/− than in wild-type chondrocytes following Fgf18 treatment.