Table V.

Survey of cell signaling proteins that show a reaction to EGF ligand stimulation over a 180-min time course

MAPK, mitogen-activated protein kinase; PTEN, phosphatase and tensin homologue deleted on chromosome 10; PDGFR, platelet-derived growth factor receptor; pT, phosphothreonine; pY, phosphotyrosine; p70S6, ribosomal protein S6 kinase.

Cell signaling protein (n = 32)/subcellular location	p value (<0.01)
	H1975 EGF-treated vs. basal			A549 EGF-treated vs. basal
	5.0 ng/ml EGF	50.0 ng/ml EGF	500 ng/ml EGF	5.0 ng/ml EGF	50.0 ng/ml EGF	500 ng/ml EGF
Membrane
EGFR Tyr-1045		<0.0001			<0.0001	<0.0001
EGFR Tyr-1068		<0.0001			<0.0001	<0.0001
EGFR Tyr-1148					0.0005	<0.0001
EGFR Tyr-1173		<0.0001	<0.0001	0.0076	<0.0001	<0.0001
EGFR Tyr-992		<0.0001	<0.0001	0.0006	<0.0001	<0.0001
HER2 Tyr-1248	0.0032	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
PDGFRβ Tyr-716	0.004	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
EGFR	0.001
c-KIT Tyr-703	0.0085	<0.0001	<0.0001			0.0014
Cytoplasm
AKT Ser-473	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
AKT Thr-308
BAD Ser-112		0.0007	0.0056		0.0042	0.0017
B-RAF Ser-445	0.0311
EIF4G Ser-1108				0.0009
ERK Thr-202/Tyr-204		0.0036	0.0074	0.0033	<0.0001	<0.0001
FAK Tyr-376		<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
FOX01 (FKHR) Thr-24					0.0029	<0.0001
GAB1 Tyr-627	0.0085	<0.0001	<0.0001		<0.0001	<0.0001
GSK3αβ Ser-21/9		<0.0001	<0.0001		<0.0001	<0.0001
LCK Tyr-505	0.0028
LKB1 Ser-428		<0.0001	<0.0001
MAPK pTEpY		<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
p70S6 Thr-389		<0.0001	<0.0001		<0.0001	<0.0001
p70S6 Thr-412		0.0005	0.003		0.0036	0.0002
PTEN Ser-380	0.0002
SHC Tyr-317	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
SRC Tyr-416		<0.0001	<0.0001		<0.0001	<0.0001
TYK2 Tyr-1054		<0.0001	<0.0001	0.0079	<0.0001	<0.0001
Nucleus
CREB Ser-133					0.003	<0.0001
ENOS III Ser-116		0.0139	0.0042
p90RSK Ser-380	0.0225	<0.0001	0.0002		0.0006	<0.0001
RSK3 Thr-356		0.0139	0.0042		0.0006	<0.0001