Fig. 4.

Colon tissue room temperature time course. Tissue specimens incubated at room temperature beyond time 0 (40 min postexcision) exhibited fluctuations in specific phosphoprotein analyte levels over time. The initial time 0 aliquot (100%) was frozen immediately, whereas the subsequent tissue aliquots were frozen at various times after room temperature incubation. White, 40 min postexcision; speckled, 45 min; hatched, 50 min; checked, 60 min; black, 70 min postexcision. Solid lines represent ±20% change from the time 0 value.