Table III.
Evaluation of post-translationally modified proteins over time for uterine leiomyoma tissue
UE, >20% increase within first 60 min postexcision; UM, >20% increase between 60 and 120 min postexcision; UL, >20% increase more than 120 min postexcision; DE, >20% decrease within first 60 min postexcision; DM, >20% decrease between 60 and 120 min postexcision; DL, >20% decrease more than 120 min postexcision.
| Protein | Function | Significant changes over time |
p values for sample 8, uterine leiomyoma (n = 24 tissue pieces), at time postexcision (time 0 = 26 min)
|
|||||
|---|---|---|---|---|---|---|---|---|
| 44 min (n = 3) | 60 min (n = 3) | 119 min (n = 4) | 146 min (n = 4) | 191 min (n = 4) | 949 min (n = 5) | |||
| Adducin (Ser-662) | Adhesion/cytoskeleton | DE/DL | 0.0013 | 0.0437 | 0.0166 | 0.002 | ||
| Annexin II | Adhesion/cytoskeleton | UL | 0.0088 | 0.0408 | ||||
| Catenin (β) (Ser-33/37/Thr-41) | Adhesion/cytoskeleton | UL | 0.0034 | 0.0278 | ||||
| E-cadherin | Adhesion/cytoskeleton | a | a | a | a | a | a | a |
| FAK (Tyr-576/577) | Adhesion/cytoskeleton | UM/JL | 0.0366 | 0.239 | ||||
| PAK1 (Ser-199/204)/PAK2 (Ser-192/197) | Adhesion/cytoskeleton | a | a | a | a | a | a | a |
| BAD (Ser-112) | Apoptosis | UM | 0.0071 | |||||
| BAX | Apoptosis | UM/UL | 0.0052 | 0.0009 | <0.0001 | 0.006 | ||
| BCL-2 (Ser-70) | Apoptosis | UL | 0.0026 | 0.0359 | ||||
| Caspase-3, cleaved (Asp-175) | Apoptosis | a | a | a | a | a | a | a |
| Caspase-7, cleaved (Asp-198) | Apoptosis | UL | 0.0029 | |||||
| Caspase-9, cleaved (Asp-330) | Apoptosis | DE | 0.0462 | 0.0029 | ||||
| CHK1 (Ser-345) | Cell cycle | UM | 0.0141 | 0.0725 | ||||
| Acetyl-CoA carboxylase (Ser-79) | Hypoxia/ischemia | UE/UM | 0.0218 | 0.0037 | ||||
| AMPKα1 (Ser-485) | Hypoxia/ischemia | UM/UL | 0.0064 | 0.002 | ||||
| AMPKβ1 (Ser-108) | Hypoxia/ischemia | UM/UL | 0.0212 | 0.0061 | 0.0002 | 0.0161 | ||
| eNOS (Ser-1177) | Hypoxia/ischemia | a | a | a | a | a | a | a |
| HIF-1α | Hypoxia/ischemia | DE/DL | 0.1043 | 0.0029 | 0.001 | |||
| HSP 90 | Hypoxia/ischemia | a | a | a | a | a | a | a |
| 4EBP1 (Ser-65) | Proliferation/survival | UL | 0.0004 | <0.0001 | 0.0001 | |||
| AKT (Ser-473) | Proliferation/survival | a | a | a | a | a | a | a |
| AKT (Thr-308) | Proliferation/survival | UL | 0.0245 | |||||
| c-ABL (Thr-735) | Proliferation/survival | DE/UM/UL | 0.0029 | 0.0103 | 0.0143 | |||
| EGFR (Tyr-1148) | Proliferation/survival | a | a | a | a | a | a | a |
| eIF4G (Ser-1108) | Proliferation/survival | a | a | a | a | a | a | a |
| ErbB2/HER2 (Tyr-1248) | Proliferation/survival | UL | 0.0015 | |||||
| ERK 1/2 (Thr-202/Tyr-204) | Proliferation/survival | UE/UM/UL | 0.0001 | 0.0002 | <0.0001 | 0.0003 | 0.0035 | |
| GSK3α (Ser-21/9) | Proliferation/survival | UL | 0.0131 | |||||
| IRS-1 (Ser-612) | Proliferation/survival | a | a | a | a | a | a | a |
| PDK1 (Ser-241) | Proliferation/survival | DE | 0.0355 | |||||
| PKCα/β II (Thr-638/641) | Proliferation/survival | a | a | a | a | a | a | a |
| Tuberin/TSC2 (Tyr-1571) | Proliferation/survival | a | a | a | a | a | a | a |
| ASK1 (Ser-83) | Stress/inflammation | UM/UL | 0.0358 | 0.0016 | 0.0044 | |||
| ATF-2 (Thr-71) | Stress/inflammation | a | a | a | a | a | a | a |
| JAK1 (Tyr-1022/1023) | Stress/inflammation | a | a | a | a | a | a | a |
| MARCKS (Ser-152/156) | Stress/inflammation | a | a | a | a | a | a | a |
| NFκB (Ser-536) | Stress/inflammation | a | a | a | a | a | a | a |
| SAP/JNK (Thr-183/Tyr-185) | Stress/inflammation | UE/UM/UL | 0.0437 | 0.0025 | <0.0001 | <0.0001 | 0.0004 | |
| SEK1/MKK4 (Ser-80) | Stress/inflammation | a | a | a | a | a | a | a |
| STAT1 (Tyr-701) | Transcription factor | UM/UL | 0.0371 | 0.0106 | ||||
| STAT3 (Tyr-705) | Transcription factor | UM/UL | <0.0001 | <0.0001 | 0.0002 | |||
a
No significant change.