Table V.
Proteins evaluated by reverse phase protein microarray in the time course studies
CST, Cell Signaling Technology; BD, BD Biosciences; Upstate, Millipore/Upstate; Biosource, Invitrogen/BIOSOURCE; A, apoptosis; B, transcription factors; C, cell cycle; D, hypoxia/ischemia; E, adhesion/cytoskeletal; F, stress/inflammation; G, proliferation/survival; FAK, focal adhesion kinase; MAPK, mitogen-activated protein kinase.
| Protein/subcellular location | Antibodyvendor/species | Function |
|---|---|---|
| Cytoplasm | ||
| 4EBP1 (Ser-65) | CST/rabbit | G |
| Acetyl-CoA carboxylase (Ser-79) | CST/rabbit | D |
| AKT (Ser-473) | CST/rabbit | G |
| AKT (Thr-308) | CST/rabbit | G |
| AMPKα1 (Ser-485) | CST/rabbit | D |
| AMPKβ (Ser-108) | CST/rabbit | D |
| ASK1 (Ser-83) | CST/rabbit | F |
| c-ABL (Thr-735) | CST/rabbit | G |
| BAD (Ser-112) | CST/rabbit | A |
| Caspase-3, cleaved (Asp-175) | CST/rabbit | A |
| Caspase-7, cleaved (Asp-198) | CST/rabbit | A |
| Caspase-9, cleaved (Asp-330) | CST/rabbit | A |
| E-cadherin | CST/rabbit | E |
| eIF4G (Ser-1108) | CST/rabbit | G |
| eNOS (Ser-1177) | CST/rabbit | D |
| ERK 1/2 (Thr-202/Tyr-204) | CST/rabbit | G |
| FAK (Tyr-576/577) | CST/rabbit | E |
| GSK3α/β (Ser-21/9) | CST/rabbit | G |
| HIF-1α | BD/mouse | D |
| HSP 90 | CST/rabbit | D |
| Iκ B-α (Ser-32/36) | CST/mouse | F |
| IRS-1 (Ser-612) | CST/rabbit | G |
| JAK1 (Tyr-1022/1023) | CST/rabbit | F |
| MARCKS (Ser-152/156) | CST/rabbit | F |
| mTOR (Ser-2481) | CST/rabbit | G |
| NFκB p65 (Ser-536) | CST/rabbit | F |
| p38 MAPK (Thr-180/Tyr-182) | CST/rabbit | D |
| PAK1 (Ser-199/204)/PAK2 (Ser-192/197) | CST/rabbit | E |
| PDK1 (Ser-241) | CST/rabbit | G |
| PKCα/β II (Thr-638/641) | CST/rabbit | G |
| SAPK/JNK (Thr-183/Tyr-183) | CST/rabbit | F |
| SEK1/MKK4 (Ser-80) | CST/rabbit | F |
| Src family (Tyr-146) | CST/rabbit | F |
| STAT1 (Tyr-701) | Upstate/rabbit | B |
| STAT3 (Ser-727) | CST/rabbit | B |
| STAT3 (Tyr-705) | CST/rabbit | B |
| Tuberin/TSC2 (Tyr-1571) | CST/rabbit | G |
| Membrane | ||
| Adducin (Ser-662) | CST/rabbit | E |
| Annexin II | BD/mouse | E |
| EGFR | CST/rabbit | G |
| EGFR (Tyr-1148) | Biosource/rabbit | G |
| ErbB2 | CST/rabbit | G |
| ErbB2/HER2 (Tyr-1248) | CST/rabbit | G |
| HER3 (Tyr-1289) | CST/rabbit | G |
| VEGF receptor-2 (Tyr-1175) | CST/rabbit | D |
| VEGFR 2 (Tyr-951) | CST/rabbit | D |
| Nucleus | ||
| ATF-2 (Thr-71) | CST/rabbit | F |
| β-Catenin (Ser-33/37/Thr-41) | CST/rabbit | E |
| CHK1 (Ser-345) | CST/rabbit | C |
| CREB (Ser-133) | CST/rabbit | B |
| Cyclin A | BD/mouse | C |
| Mitochondria | ||
| BCL-2 (Ser-70) | CST/rabbit | A |
| BAX | CST/rabbit | A |