Table 1.
SE analysis of molecular mass as a function of pea PBGS protein concentration, assuming a single ideal species
| Sample/Speed1 | 8,000 rpm | 10,000 rpm | 12,000 rpm | Global2 |
|---|---|---|---|---|
| 30 μM | 266,5003 | 257,000 | 253,000 | 257,000±7,500 |
| 10 μM | 274,000 | 272,000 | 273,000 | 273,200±8,000 |
| 3 μM | 269,000 | 273,000 | 266,000 | 269,000±10,000 |
| all conc’ns4 | 270,000 | 263,000 | 259,500 | 264,000±5,500 |
1
0.1 M BTP-HCl, pH 8.5, 0.1 mM DTT, 10 mM MgCl2 collected at 20°C.
2
The term, “global” refers to global fits across all speeds for a given concentration. The last row of this column represents an global fit to all three speeds and three concentrations simultaneously.
3
All molecular masses are reported in Daltons. The theoretical monomer molecular mass of pea PBGS is 36,693 Da.
4
The row labeled “all conc’ns” contains global fits across all concentrations for a given speed.