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A Sensitive Reporter System For miR-277 Silencing. Clonal Drosophila S2 cell lines bearing stably integrated GFP transgenes containing no sites, two perfect or four bulged miR-277-complementary sites in the 3′ UTR were transfected with control or miR-277-complementary ASOs (33 nt 2′-O-Me bearing a 3′ cholesterol modification). Five days after transfection, GFP was measured by FACS.
