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. Author manuscript; available in PMC: 2008 Oct 3.
Published in final edited form as: Nat Protoc. 2008;3(10):1537–1549. doi: 10.1038/nprot.2008.145

Table 1.

Cell, ASO, and Lipid Volumes for Transfection of Cultured Drosophila Cells

Volume of cells (2.75 × 105/ml) ASO mix Lipid mix
ASO (10 μM) Schneider media Dharmafect 4 Schneider media
24 well plate 450 μl / well 1.25 μl 23.75 μl 1 μl 24 μl
6 well plate 1.8 ml / well 5 μl 95 μl 4 μl 94 μl
10 cm Dish 9 ml / dish 25 μl 475 μl 20 μl 480 μl
HHS Vulnerability Disclosure